human tnbc cell lines hcc38  (ATCC)

Journal: Cell Death Discovery

Article Title: Repurposing proteasome inhibitors for improved treatment of triple-negative breast cancer

doi: 10.1038/s41420-024-01819-5

Figure Lengend Snippet: A and B show the efficiency of the 18 drugs on eight TNBC cell lines (BT-549, CAL-148, HCC1806, HCC38, HCC70, MDA-MB-436, MDA-MB-453, and MDA-MB-468) and two controls (MCF-10A and MCF-7) using growth rate inhibition (GR 50 ) and area under the curve (AUC). Bortezomib, carfilzomib, cisplatin, delanzomib, docetaxel, epoxomicin, MLN-2238, MLN-9708 and nedaplatin were shown to be potent drugs (GR 50 < 1000 nM and AUC < 0.80). C Scatterplot illustrating cytotoxicity with GR max and GR 50 values. Topoisomerases were shown to be weak inhibitors, while proteasome inhibitors, mitosis inhibitors, and platinum agents had an adverse effect on cell viability. D and E show the cytotoxicity of all 18 drugs at the highest tested dose. The most cytotoxic drugs were celastrol, cisplatin, and nedaplatin, and the most sensitive cell lines to the highest dose were HCC38, HCC70, and MDA-MB-436. F show expression of AR in the 10 cell lines. Cell lines BT-549 (M), CAL-148 (LAR), HCC70 (BL1) and MDA-MB-453 (LAR) were AR-positive. Elevated AR expression was associated with high GR 50 values.

Article Snippet: Eight human TNBC cell lines (BT-549, CAL-148, HCC38, HCC70, HCC1806, MDA-MB-436, MDA-MB-453, and MDA-MB-468) and four control cell lines (BT-474, MCF-7, MCF-10A, and T47D) were purchased from American Type Culture Collection (ATCC) or German Collection of Microorganisms and Cell Cultures GmbH (DSMZ), and authenticated using the Eurofins Genomics Human Cell Line Authentication Service.

Techniques: Inhibition, Expressing